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Impact of PMAxx and <t>PMA</t> <t>Enhancer</t> (Enhancer) on E. coli cell viability. ( A ) Viability of E. coli cells was assessed using PD-cDDLAMP with a signal-off detection approach following treatment with PMAxx, Enhancer, a combination of both, or no treatment. The signal-to-noise ratio (S/N) was calculated as the ratio of the OD (optical density at 655 nm) for the positive sample to the no-template control, with buffer background OD subtracted from both values. ( B ) Comparison of the ∆Ct values in qPCR between PMAxx alone, combined PMAxx and enhancer treatment, and untreated genomic DNA samples. ∆Ct is defined as the Ct difference between treated and untreated samples for both live and dead E. coli cells. Raw Ct values are provided in . Error bars represent the standard error of two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.
Pma Enhancer, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gram-negative+bacteria/PMA+Enhancer+for+Gram+Negative+Bacteria/pmc13689256-72-1-3
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gram negative bacteria  (ATCC)
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Impact of PMAxx and <t>PMA</t> <t>Enhancer</t> (Enhancer) on E. coli cell viability. ( A ) Viability of E. coli cells was assessed using PD-cDDLAMP with a signal-off detection approach following treatment with PMAxx, Enhancer, a combination of both, or no treatment. The signal-to-noise ratio (S/N) was calculated as the ratio of the OD (optical density at 655 nm) for the positive sample to the no-template control, with buffer background OD subtracted from both values. ( B ) Comparison of the ∆Ct values in qPCR between PMAxx alone, combined PMAxx and enhancer treatment, and untreated genomic DNA samples. ∆Ct is defined as the Ct difference between treated and untreated samples for both live and dead E. coli cells. Raw Ct values are provided in . Error bars represent the standard error of two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.
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gram negative bacteria  (Biotium)
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Impact of PMAxx and <t>PMA</t> <t>Enhancer</t> (Enhancer) on E. coli cell viability. ( A ) Viability of E. coli cells was assessed using PD-cDDLAMP with a signal-off detection approach following treatment with PMAxx, Enhancer, a combination of both, or no treatment. The signal-to-noise ratio (S/N) was calculated as the ratio of the OD (optical density at 655 nm) for the positive sample to the no-template control, with buffer background OD subtracted from both values. ( B ) Comparison of the ∆Ct values in qPCR between PMAxx alone, combined PMAxx and enhancer treatment, and untreated genomic DNA samples. ∆Ct is defined as the Ct difference between treated and untreated samples for both live and dead E. coli cells. Raw Ct values are provided in . Error bars represent the standard error of two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.
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https://www.bioz.com/product/gram-negative+bacteria/PMA+Enhancer+for+Gram+Negative+Bacteria/pm41903231-87-16-21
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Impact of PMAxx and <t>PMA</t> <t>Enhancer</t> (Enhancer) on E. coli cell viability. ( A ) Viability of E. coli cells was assessed using PD-cDDLAMP with a signal-off detection approach following treatment with PMAxx, Enhancer, a combination of both, or no treatment. The signal-to-noise ratio (S/N) was calculated as the ratio of the OD (optical density at 655 nm) for the positive sample to the no-template control, with buffer background OD subtracted from both values. ( B ) Comparison of the ∆Ct values in qPCR between PMAxx alone, combined PMAxx and enhancer treatment, and untreated genomic DNA samples. ∆Ct is defined as the Ct difference between treated and untreated samples for both live and dead E. coli cells. Raw Ct values are provided in . Error bars represent the standard error of two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.
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https://www.bioz.com/product/gram-negative+bacteria/Bacteria/us12577525-175-0-14
Average 99 stars, based on 1 article reviews
i preferred gram negative bacteria - by Bioz Stars, 2026-11
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gram negative bacteria  (System Biosciences Inc)
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Impact of PMAxx and <t>PMA</t> <t>Enhancer</t> (Enhancer) on E. coli cell viability. ( A ) Viability of E. coli cells was assessed using PD-cDDLAMP with a signal-off detection approach following treatment with PMAxx, Enhancer, a combination of both, or no treatment. The signal-to-noise ratio (S/N) was calculated as the ratio of the OD (optical density at 655 nm) for the positive sample to the no-template control, with buffer background OD subtracted from both values. ( B ) Comparison of the ∆Ct values in qPCR between PMAxx alone, combined PMAxx and enhancer treatment, and untreated genomic DNA samples. ∆Ct is defined as the Ct difference between treated and untreated samples for both live and dead E. coli cells. Raw Ct values are provided in . Error bars represent the standard error of two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.
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gram  (ATCC)
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ATCC gram
Impact of PMAxx and <t>PMA</t> <t>Enhancer</t> (Enhancer) on E. coli cell viability. ( A ) Viability of E. coli cells was assessed using PD-cDDLAMP with a signal-off detection approach following treatment with PMAxx, Enhancer, a combination of both, or no treatment. The signal-to-noise ratio (S/N) was calculated as the ratio of the OD (optical density at 655 nm) for the positive sample to the no-template control, with buffer background OD subtracted from both values. ( B ) Comparison of the ∆Ct values in qPCR between PMAxx alone, combined PMAxx and enhancer treatment, and untreated genomic DNA samples. ∆Ct is defined as the Ct difference between treated and untreated samples for both live and dead E. coli cells. Raw Ct values are provided in . Error bars represent the standard error of two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.
Gram, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gram - by Bioz Stars, 2026-11
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Impact of PMAxx and PMA Enhancer (Enhancer) on E. coli cell viability. ( A ) Viability of E. coli cells was assessed using PD-cDDLAMP with a signal-off detection approach following treatment with PMAxx, Enhancer, a combination of both, or no treatment. The signal-to-noise ratio (S/N) was calculated as the ratio of the OD (optical density at 655 nm) for the positive sample to the no-template control, with buffer background OD subtracted from both values. ( B ) Comparison of the ∆Ct values in qPCR between PMAxx alone, combined PMAxx and enhancer treatment, and untreated genomic DNA samples. ∆Ct is defined as the Ct difference between treated and untreated samples for both live and dead E. coli cells. Raw Ct values are provided in . Error bars represent the standard error of two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.

Journal: Microbiology Spectrum

Article Title: PMAxx dye-assisted colorimetric dual DNAzyme-LAMP (PD-cDDLAMP) for rapid phenotypic antimicrobial susceptibility testing of Escherichia coli

doi: 10.1128/spectrum.01212-26

Figure Lengend Snippet: Impact of PMAxx and PMA Enhancer (Enhancer) on E. coli cell viability. ( A ) Viability of E. coli cells was assessed using PD-cDDLAMP with a signal-off detection approach following treatment with PMAxx, Enhancer, a combination of both, or no treatment. The signal-to-noise ratio (S/N) was calculated as the ratio of the OD (optical density at 655 nm) for the positive sample to the no-template control, with buffer background OD subtracted from both values. ( B ) Comparison of the ∆Ct values in qPCR between PMAxx alone, combined PMAxx and enhancer treatment, and untreated genomic DNA samples. ∆Ct is defined as the Ct difference between treated and untreated samples for both live and dead E. coli cells. Raw Ct values are provided in . Error bars represent the standard error of two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.

Article Snippet: 5× PMA Enhancer (Biotium) was diluted to a 1× final concentration by adding 50 μL to 200 μL of bacterial suspension mixed with tetracycline.

Techniques: Control, Comparison, Analysis

Impact of PMAxx and PMA Enhancer (Enh) on E. coli susceptibility to AMP and TET. Signal-on and off detection approaches for antibiotic-susceptible and -resistant E. coli strains upon exposure to AMP ( A ) and TET ( B ) were evaluated using PD-cDDLAMP. Cells were treated with either PMAxx alone or PMAxx plus the enhancer. Heat-lysed dead and live cells served as controls. The signal-to-noise ratio (S/N) was determined by calculating the ratio of the OD (optical density at 655 nm) for each positive sample relative to the no-template control, with buffer background OD subtracted from both values. Error bars represent the standard error from two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.

Journal: Microbiology Spectrum

Article Title: PMAxx dye-assisted colorimetric dual DNAzyme-LAMP (PD-cDDLAMP) for rapid phenotypic antimicrobial susceptibility testing of Escherichia coli

doi: 10.1128/spectrum.01212-26

Figure Lengend Snippet: Impact of PMAxx and PMA Enhancer (Enh) on E. coli susceptibility to AMP and TET. Signal-on and off detection approaches for antibiotic-susceptible and -resistant E. coli strains upon exposure to AMP ( A ) and TET ( B ) were evaluated using PD-cDDLAMP. Cells were treated with either PMAxx alone or PMAxx plus the enhancer. Heat-lysed dead and live cells served as controls. The signal-to-noise ratio (S/N) was determined by calculating the ratio of the OD (optical density at 655 nm) for each positive sample relative to the no-template control, with buffer background OD subtracted from both values. Error bars represent the standard error from two independent experiments, each with four replicates ( n = 4). Statistical analysis is denoted as **** P < 0.0001, ns, P > 0.05.

Article Snippet: 5× PMA Enhancer (Biotium) was diluted to a 1× final concentration by adding 50 μL to 200 μL of bacterial suspension mixed with tetracycline.

Techniques: Control, Analysis